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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
19/07/2023 |
Actualizado : |
19/07/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
SANTANA, A.; CAJARVILLE, C.; MENDOZA, A.; REPETTO, J.L. |
Afiliación : |
A. SANTANA, Departamento de Producción Animal y Salud de los Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, San José, 801000, Uruguay; CECILIA CAJARVILLE, Departamento de Producción Animal y Salud de los Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, San José, 801000, Uruguay; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ LUIS REPETTO, Departamento de Producción Animal y Salud de los Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, San José, 801000, Uruguay. |
Título : |
Including 8 hours of access to alfalfa in 1 or 2 grazing sessions in dairy cows fed a partial mixed ration: Effects on intake, behavior, digestion, and milk production and composition. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Journal of Dairy Science. 2023, https://doi.org/10.3168/jds.2022-22743 --- [In Press, Corrected Proof]. OPEN ACCESS. |
ISSN : |
0022-0302 |
DOI : |
10.3168/jds.2022-22743 |
Idioma : |
Inglés |
Notas : |
Article history: Received 8 September 2022; Accepted 3 March 2023; Available online 18 July 2023. -- Correspondence author: : joselorepetto@gmail.com -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- FUNDING: This experiment was partially supported by the Agencia Nacional de Innovación e Investigación of Uruguay (Grant FMV_3-2018-1-148846 to A. Santana). -- |
Contenido : |
The aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk composition, and milk fatty acid profile, in late-lactation cows fed a partial mixed ration (PMR). Twelve dairy cows (193 ± 83 d in milk, 584 ± 71 kg of body weight) were housed in individual outdoor pens and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. The treatments were as follows: (1) control (T0), cows were fed a total mixed ration (TMR) provided ad libitum 20.0% crude protein (CP), 32.2% neutral detergent fiber (NDF); (2) fed a diet combining a PMR which had the same ingredient composition as the TMR (60% of ad libitum intake) + 1 session of 8 h of pasture (T8), continuous grazing alfalfa (Medicago sativa; 20.6% CP, 35.8% NDF) after the p.m. milking; and (3) PMR (60% of ad libitum intake) + 2 daily sessions of 4 h of access to pasture after each milking (T4+4). The experiment lasted 57 d and was divided into 3 periods of 19 d each. The first 12 d of each period was used for diet adaptation, and the last 7 d was used for data collection. No differences among treatments were observed for any of the productive variables, feeding efficiency, or purine derivatives excretion. Cows in T0 had greater intake and apparent digestibility of dry matter, organic matter, and nonfibrous carbohydrates compared with T4+4 and T8. Compared with T0, alfalfa grazing increased the concentration of C18:1 trans-11 and decreased those of C16:0 and C17:0 in milk fat. Cows in T4+4 consumed 1.1 more kg DM/d of alfalfa and N provided by alfalfa in the diet was 3 percentage points higher compared with T8 cows (266 vs. 229 g/d, respectively). In addition, T4+4 cows had a greater daily range of ruminal pH than T8 (0.73 vs. 0.93), and the highest concentrations of NH3-N were recorded during the a.m. grazing session while in T8 cows it occurred during the night. In conclusion, including 8 h of alfalfa grazing in T8 and T4+4 treatments allowed the substitution between 35.8 and 38.7% of the total dry matter intake (DMI) of a PMR (with a similar CP concentration to alfalfa) for pasture, maintaining milk solids production and increasing the C18:1 trans-11 of milk fat compared with a TMR in mid late-lactation cows. In an herbage plus PMR diet, splitting the 1 continuous grazing session of 8 h into 2 sessions of 4 h increased the proportion of energy and N provided by alfalfa pasture and reduced PMR intake, without modifying the total nutrient intake or productive performance of cows. © 2023, The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association® MenosThe aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk composition, and milk fatty acid profile, in late-lactation cows fed a partial mixed ration (PMR). Twelve dairy cows (193 ± 83 d in milk, 584 ± 71 kg of body weight) were housed in individual outdoor pens and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. The treatments were as follows: (1) control (T0), cows were fed a total mixed ration (TMR) provided ad libitum 20.0% crude protein (CP), 32.2% neutral detergent fiber (NDF); (2) fed a diet combining a PMR which had the same ingredient composition as the TMR (60% of ad libitum intake) + 1 session of 8 h of pasture (T8), continuous grazing alfalfa (Medicago sativa; 20.6% CP, 35.8% NDF) after the p.m. milking; and (3) PMR (60% of ad libitum intake) + 2 daily sessions of 4 h of access to pasture after each milking (T4+4). The experiment lasted 57 d and was divided into 3 periods of 19 d each. The first 12 d of each period was used for diet adaptation, and the last 7 d was used for data collection. No differences among treatments were observed for any of the productive variables, feeding efficiency, or purine derivatives excretion. Cows in T0 had greater intake and apparent digestibility of dry matter, organic matter, and nonfibrous carbohyd... Presentar Todo |
Palabras claves : |
Fresh forage; Partial mixed ration; Ruminal fermentation. |
Thesagro : |
ALFALFA. |
Asunto categoría : |
L02 Alimentación animal |
URL : |
https://www.sciencedirect.com/science/article/pii/S0022030223003740/pdf
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Marc : |
LEADER 04084naa a2200241 a 4500 001 1064258 005 2023-07-19 008 2023 bl uuuu u00u1 u #d 022 $a0022-0302 024 7 $a10.3168/jds.2022-22743$2DOI 100 1 $aSANTANA, A. 245 $aIncluding 8 hours of access to alfalfa in 1 or 2 grazing sessions in dairy cows fed a partial mixed ration$bEffects on intake, behavior, digestion, and milk production and composition.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 8 September 2022; Accepted 3 March 2023; Available online 18 July 2023. -- Correspondence author: : joselorepetto@gmail.com -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- FUNDING: This experiment was partially supported by the Agencia Nacional de Innovación e Investigación of Uruguay (Grant FMV_3-2018-1-148846 to A. Santana). -- 520 $aThe aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk composition, and milk fatty acid profile, in late-lactation cows fed a partial mixed ration (PMR). Twelve dairy cows (193 ± 83 d in milk, 584 ± 71 kg of body weight) were housed in individual outdoor pens and assigned to treatments according to a 3 × 3 Latin square design replicated 4 times. The treatments were as follows: (1) control (T0), cows were fed a total mixed ration (TMR) provided ad libitum 20.0% crude protein (CP), 32.2% neutral detergent fiber (NDF); (2) fed a diet combining a PMR which had the same ingredient composition as the TMR (60% of ad libitum intake) + 1 session of 8 h of pasture (T8), continuous grazing alfalfa (Medicago sativa; 20.6% CP, 35.8% NDF) after the p.m. milking; and (3) PMR (60% of ad libitum intake) + 2 daily sessions of 4 h of access to pasture after each milking (T4+4). The experiment lasted 57 d and was divided into 3 periods of 19 d each. The first 12 d of each period was used for diet adaptation, and the last 7 d was used for data collection. No differences among treatments were observed for any of the productive variables, feeding efficiency, or purine derivatives excretion. Cows in T0 had greater intake and apparent digestibility of dry matter, organic matter, and nonfibrous carbohydrates compared with T4+4 and T8. Compared with T0, alfalfa grazing increased the concentration of C18:1 trans-11 and decreased those of C16:0 and C17:0 in milk fat. Cows in T4+4 consumed 1.1 more kg DM/d of alfalfa and N provided by alfalfa in the diet was 3 percentage points higher compared with T8 cows (266 vs. 229 g/d, respectively). In addition, T4+4 cows had a greater daily range of ruminal pH than T8 (0.73 vs. 0.93), and the highest concentrations of NH3-N were recorded during the a.m. grazing session while in T8 cows it occurred during the night. In conclusion, including 8 h of alfalfa grazing in T8 and T4+4 treatments allowed the substitution between 35.8 and 38.7% of the total dry matter intake (DMI) of a PMR (with a similar CP concentration to alfalfa) for pasture, maintaining milk solids production and increasing the C18:1 trans-11 of milk fat compared with a TMR in mid late-lactation cows. In an herbage plus PMR diet, splitting the 1 continuous grazing session of 8 h into 2 sessions of 4 h increased the proportion of energy and N provided by alfalfa pasture and reduced PMR intake, without modifying the total nutrient intake or productive performance of cows. © 2023, The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association® 650 $aALFALFA 653 $aFresh forage 653 $aPartial mixed ration 653 $aRuminal fermentation 700 1 $aCAJARVILLE, C. 700 1 $aMENDOZA, A. 700 1 $aREPETTO, J.L. 773 $tJournal of Dairy Science. 2023, https://doi.org/10.3168/jds.2022-22743 --- [In Press, Corrected Proof]. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
23/04/2015 |
Actualizado : |
23/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 1 |
Autor : |
LADO, J.; ALÓS, E.; RODRIGO, M.J.; ZACARÍAS, L. |
Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUETA ALÓS, CSIC/ IAS (Instituto de Agricultura Sostenible); MARÍA JESÚS RODRIGO, IATA (Instituto de Agroquímica y Tecnología de Alimentos); LORENZO ZACARÍAS, IATA (Instituto de Agroquímica y Tecnología de Alimentos). |
Título : |
Light avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Plant Science, 2015, v.231, p.138-147. |
DOI : |
10.1016/j.plantsci.2014.12.002 |
Idioma : |
Inglés |
Notas : |
Article history: Received 2 October 2014 / Received in revised form 26 November 2014 / Accepted 1 December 2014 / Available online 5 December 2014. |
Contenido : |
ABSTRACT.
Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue. |
Palabras claves : |
VITAMINA C. |
Thesagro : |
ANTIOXIDANTES; CITRUS; EXPRESION GENICA; VITAMINAS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02127naa a2200241 a 4500 001 1052574 005 2019-10-23 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.plantsci.2014.12.002$2DOI 100 1 $aLADO, J. 245 $aLight avoidance reduces ascorbic acid accumulation in the peel of Citrus fruit.$h[electronic resource] 260 $c2015 500 $aArticle history: Received 2 October 2014 / Received in revised form 26 November 2014 / Accepted 1 December 2014 / Available online 5 December 2014. 520 $aABSTRACT. Citrus fruits are highly consumed worldwide and represent one of the most important sources of ascorbic acid (AsA). However, information about the molecular mechanisms regulating AsA accumulation in Citrus fruit and the effects of environmental factors is scarce. In this study we have investigated the effect of fruit shading on AsA content and the expression of AsA biosynthetic, degrading and recycling genes in fruits of different Citrus species. Immature-green fruits were covered at the end of the cell enlargement phase and AsA concentration in the flavedo declined and remained at low levels as compared with light-exposed fruits. Fruit shading marginally altered the expression of genes from the l-galactose pathway and this effect was variable in the four Citrus species. However, specific isoforms (GalUR8 or GalUR12) from the l-galacturonic acid pathway were significantly repressed paralleling the reduction in AsA concentration. No significant effect of shading was detected in transcription of genes of the myo-inositol and l-gulose pathways as well as recycling and degradation. Collectively, results indicate that light avoidance inhibited accumulation of AsA in the flavedo of Citrus fruits and suggest that the l-galacturonic acid pathway has a relevant contribution to AsA content in this tissue. 650 $aANTIOXIDANTES 650 $aCITRUS 650 $aEXPRESION GENICA 650 $aVITAMINAS 653 $aVITAMINA C 700 1 $aALÓS, E. 700 1 $aRODRIGO, M.J. 700 1 $aZACARÍAS, L. 773 $tPlant Science, 2015$gv.231, p.138-147.
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